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KMID : 0358419960390010102
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Korean Journal of Obstetrics and Gynecology
1996 Volume.39 No. 1 p.102 ~ p.112
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김정구
김석현/최영민/신창재/문신용/장윤석/이진용
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Abstract
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Uterine leiomyomata are the most common pelvic tumor in women but their etiology is unknown. Estradiol is believed to a stimulus for their growth, acting via the insulin-like growth factors(IGFs's, potent mitogenic peptides. IGP-binding
proteins(IGFBPs)
may modulate IGF actions at their target cells and directly affect the function of these cells. The purpose of this study is to compare IGFBP profiles in uterine leiomyoma and adjacent normal myometrium and to evaluate the effect of 17
β-estradiol
on
the production of IGFBPs in these explant cultures. IGFBP-s, IGFBP-3 and 26kDa IGFBP were detected variably in tissue cytosols from uterine leiomyoma and adjacent normal myometrium and in conditioned media of explant cultures by western lignad
blotting
and immunoprecipitation, but IGFBP-4 were consistenly present. There were no differences in the relative intensity for IGFBP-4 and the frequency of each IGFBP between leiomyoma and adjacent normal myometrium. For each patient the complement of
IGFBPs
was similar in tissue cytosols and conditioned media of explants.
Treatment of leiomyoma explants with 17 β-estradiol resulted in significant increase in the intensity of IGFBP-4 in conditioned media when compared to the control condition whereas this treatment did not affect IGFBP-4 production in normal
myometrial
explants. These data support that IGFBP-4 may be a mediator of 17β-estradiol action in these tissue and that there may be an enhanced sensitivity toward IGFBP-4 production in leiomyoma compared to adjacent normal myometrium.
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KEYWORD
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